Article
Polymorphism of the ovine calpastatin gene.
Molecular and cellular probes - 1 Jun 2007
Zhou H, Hickford J G H, Gong H
Abstract excerpt
Calpastatin is a specific inhibitor of calpains and has been implicated in the regulation of beef tenderization. Variation in the ovine calpastatin gene (CAST) was investigated by amplification of a fragment containing the entire exon 6 using polymerase chain reaction (PCR), followed by single-strand conformational polymorphism (PCR-SSCP) analysis and DNA sequencing. Five novel SSCP patterns, representing five...
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