Article
A consensus motif common to all Rho-dependent prokaryotic transcription terminators.
Cell - 8 Feb 1991
Alifano P, Rivellini F, Limauro D, Bruni C B, Carlomagno M S
Abstract excerpt
We have characterized at the molecular level several polar mutations in four different cistrons of the his operon of S. typhimurium. An analysis of the his-specific transcripts produced in vivo in the mutant strains, together with in vitro transcription assays, led to the identification of several cryptic Rho-dependent transcription termination elements within the his operon that are activated by the uncoupling...
Topics
- Base Sequence
- Blotting, Northern
- Cloning, Molecular
- DNA, Bacterial
- Histidine
- Molecular Sequence Data
- Mutation
- Operon
- RNA, Bacterial
- RNA, Messenger
- Regulatory Sequences, Nucleic Acid