Article
New PCR systems to confirm real-time PCR detection of Mycobacterium avium subsp. paratuberculosis.
1 Jan 2006
Abstract excerpt
BACKGROUND: Johne's disease, a serious chronic form of enteritis in ruminants, is caused by Mycobacterium avium subsp. paratuberculosis (MAP). As the organism is very slow-growing and fastidious, several PCR-based methods for detection have been developed, based mainly on the MAP-specific gene IS900. However, because this gene is similar to genes in other mycobacteria, there is a need for sensitive and reliable...
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