Article
Characterization of chromosomal DNA amplifications with associated tetracycline resistance in Bacillus subtilis.
Journal of bacteriology - 1 Sept 1990
Ives C L, Bott K F
Abstract excerpt
Endogenous chromosomal DNA amplifications with associated tetracycline resistance (Tcr) in Bacillus subtilis were first described by C. R. Wilson and A. E. Morgan (J. Bacteriol. 163:445-453, 1985). We have confirmed and extended their results, and we show that fusion of protoplasts from Tcs B. subtilis 168 trpC2 with polyethylene glycol and regeneration on medium containing 20 micrograms of tetracycline per ml...
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