Article
Isolation, phenotyping, and functional analysis of lymphocytes from human liver.
Clinical immunology and immunopathology - 1 Sept 1990
Hata K, Zhang X R, Iwatsuki S, Van Thiel D H, Herberman R B, Whiteside T L
Abstract excerpt
The isolation of mononuclear cells from human liver tissues was achieved by a simple method consisting of enzymatic and mechanical dissociation, density gradient centrifugation, and adherence to plastic. The method was optimized to obtain a nearly complete recovery of different lymphoid subpopulations. The mononuclear cells recovered from "normal" liver tissues consisted of 33 +/- 9% (mean +/- SD) small...
Topics
- Aged
- Antigens, Differentiation, T-Lymphocyte
- CD3 Complex
- CD4 Antigens
- CD56 Antigen
- Cell Separation
- Cytotoxicity, Immunologic
- Flow Cytometry
- Humans
- Killer Cells, Natural
- Liver