Article
Isolation, phenotyping, and functional analysis of lymphocytes from human liver.
Clinical immunology and immunopathology - 1 Sept 1990
Hata K, Zhang X R, Iwatsuki S, Van Thiel D H, Herberman R B, Whiteside T L
Abstract excerpt
The isolation of mononuclear cells from human liver tissues was achieved by a simple method consisting of enzymatic and mechanical dissociation, density gradient centrifugation, and adherence to plastic. The method was optimized to obtain a nearly complete recovery of different lymphoid subpopulations. The mononuclear cells recovered from "normal" liver tissues consisted of 33 +/- 9% (mean +/- SD) small...
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