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Article

Ex Vivo Large‐Scale Generation of Human Platelets from Cord Blood CD34 <sup>+</sup> Cells

8 Sept 2006

Abstract excerpt

In the present investigation, we generated platelets (PLTs) from cord blood (CB) CD34(+) cells using a three-phase culture system. We first cultured 500 CB CD34(+) cells on telomerase gene-transduced human stromal cells (hTERT stroma) in serum-free medium supplemented with stem cell factor (SCF), Flt-3/Flk-2 ligand (FL), and thrombopoietin (TPO) for 14 days. We then transferred the cells to hTERT stroma and...

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Identifiers

PMID: 16960134
DOI: 10.1634/stemcells.2006-0309

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