Article
Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale.
Applied and environmental microbiology - 1 Sept 2006
Aminov Rustam I, Walker Alan W, Duncan Sylvia H, Harmsen Hermie J M, Welling Gjalt W, Flint Harry J
Abstract excerpt
Phylogenetic analysis was used to compare 16S rRNA sequences from 19 cultured human gut strains of Roseburia and Eubacterium rectale with 356 related sequences derived from clone libraries. The cultured strains were found to represent five of the six phylotypes identified. A new oligonucleotide probe, Rrec584, and the previous group probe Rint623, when used in conjunction with a new helper oligonucleotide, each...
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