Article
An element of the tertiary structure of Peach latent mosaic viroid RNA revealed by UV irradiation.
Journal of virology - 1 Sept 2006
Hernández Carmen, Di Serio Francesco, Ambrós Silvia, Daròs José-Antonio, Flores Ricardo
Abstract excerpt
Following UV irradiation, denaturing polyacrylamide gel electrophoresis and Northern blot hybridization revealed a cross-link in Peach latent mosaic viroid (PLMVd) plus-strand RNA. Primer extension and partial alkaline hydrolysis of the UV-irradiated PLMVd plus-strand RNA resulting from the hammerhead-mediated self-cleavage mapped the cross-link at U81 and at the 3'-terminal C289 (or at a very proximal...
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