Evaluation of a PCR Melting Profile Technique for Bacterial Strain Differentiation

Abstract excerpt

In search of an effective DNA typing technique for hospital epidemiology use, the performance and convenience of a PCR melting profile (PCR MP) technique based on using low denaturation temperatures during ligation-mediated PCR (LM PCR) of bacterial DNA was tested. A number of Escherichia coli isolates from patients of the Clinical Hospital in Gdańsk, Poland, were examined. We found that the PCR MP technique is a...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Bacteriophages and microbial interactions
• Share to Molecular Biology Techniques and Applications
• Share to Mycobacterium research and diagnosis