Article
Genetic heterogeneity of urease gene loci in urease-positive thermophilic Campylobacter (UPTC).
International journal of hygiene and environmental health - 1 Nov 2006
Usui Kaori, Iida Haruna, Ueno Hitomi, Sekizuka Tsuyoshi, Matsuda Motoo, Murayama Ohoshi, Cherie Millar B, Moore John E
Abstract excerpt
Degenerate PCR primers in silico based on the two urease structural genes, ureA and ureB, were designed for urease-positive thermophilic Campylobacter (UPTC). Resultant PCR amplification employing these primers generated an amplicon of approximately 2kb, which was cloned and sequenced in UPTC (n=12) isolated from various parts of Europe and Japan. Overall, sequence similarities were shown to be 96.7 to 99.9%....
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