Article
Internally controlled real-time PCR method for quantitative species-specific detection and vapA genotyping of Rhodococcus equi.
Applied and environmental microbiology - 1 Jun 2006
Rodríguez-Lázaro David, Lewis Deborah A, Ocampo-Sosa Alain A, Fogarty Ursula, Makrai László, Navas Jesús, Scortti Mariela, Hernández Marta, Vázquez-Boland José A
Abstract excerpt
We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. Species-specific quantification was achieved by targeting the chromosomal monocopy gene choE, universally conserved in R. equi. The choE Q-PCR included an internal amplification control (IAC) for identification of false negatives. A second Q-PCR...
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