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Sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine semen by real-time PCR

12 Apr 2006

Abstract excerpt

AIMS: To develop a fast and sensitive protocol for detection of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine semen and to make a critical evaluation of the analytical sensitivity. METHODS AND RESULTS: Processed semen was spiked with known amounts of MAP. Semen from different bulls as well as semen of different dilutions was tested. The samples were treated with lysing agents and beadbeating and the...

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PMID: 16630010
DOI: 10.1111/j.1365-2672.2006.02924.x

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