A Comparison of Morphologic Features, Flow Cytometry, TCR-V_βAnalysis, and TCR-PCR in Qualitative and Quantitative Assessment of Peripheral Blood Involvement by Sézary Syndrome

Abstract excerpt

The strengths and weaknesses of various laboratory methods for peripheral blood (PB) Sézary cell quantitation have not been compared rigorously. In this study, manual Sézary cell counting, qualitative and quantitative flow cytometry, T-cell receptor (TCR) Vbeta flow cytometry, and TCR polymerase chain reaction were performed on PB specimens from 11 patients with Sézary syndrome (SS), 9 with reactive erythroderma,...

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