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Secretory Protein Enrichment and Analysis: An Optimized Approach Applied on Cancer Cell Lines Using 2D LC−MS/MS

10 Mar 2006

Abstract excerpt

Reliable methods for profiling secretory proteins are highly desirable for the identification of biomarkers of disease progression. Secreted proteins are often masked by high amounts of protein supplements in the culture medium. We have developed an efficient method for the enrichment and analysis of the secretome of different cancer cell lines, free of essential contaminants. The method is based on the...

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PMID: 16602697
DOI: 10.1021/pr050375p

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