Article
Reactivation of a silent Ac following tissue culture is associated with heritable alterations in its methylation pattern.
Molecular & general genetics : MGG - 1 Oct 1991
Brettell R I, Dennis E S
Abstract excerpt
Tissue cultures were initiated from embryos with an inactive form of Ac in the wx-m9 Ds-cy allele. Plants regenerated from the cultures showed a high frequency of activation of Ac. That activation was shown to be associated with reduced methylation of cytosine residues at the 5' end of the transposable element. An examination of Ac activity and methylation status of the Ac element in progenies of the regenerant...
Topics
- Alleles
- Culture Techniques
- DNA
- DNA Transposable Elements
- Gene Expression Regulation
- Methylation
- Restriction Mapping
- Zea mays