Article
Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay.
BMC microbiology - 9 Feb 2006
Le Flèche Philippe, Jacques Isabelle, Grayon Maggy, Al Dahouk Sascha, Bouchon Patrick, Denoeud France, Nöckler Karsten, Neubauer Heinrich, Guilloteau Laurence A, Vergnaud Gilles
Abstract excerpt
BACKGROUND: The classification of Brucella into species and biovars relies on phenotypic characteristics and sometimes raises difficulties in the interpretation of the results due to an absence of standardization of the typing reagents. In addition, the resolution of this biotyping is moderate and requires the manipulation of the living agent. More efficient DNA-based methods are needed, and this work explores...
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