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Evaluation of Three Diagnostic Methods, Including Real-Time PCR, for Detection of <i>Dientamoeba fragilis</i> in Stool Specimens

1 Jan 2006

Abstract excerpt

Dientamoeba fragilis is a protozoan parasite of humans that infects the mucosa of the large intestine and is associated with gastrointestinal disease. We developed a 5' nuclease (TaqMan)-based real-time PCR assay, targeting the small subunit rRNA gene, for the detection of D. fragilis in human stool specimens and compared its sensitivity and specificity to conventional PCR and microscopic examination by a...

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PMID: 16390978
DOI: 10.1128/jcm.44.1.232-235.2006

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