Article
Gene silencing of cinnamyl alcohol dehydrogenase in Pinus radiata callus cultures.
Plant physiology and biochemistry : PPB - 1 Dec 2005
Möller Ralf, Steward Diane, Phillips Lorelle, Flint Heather, Wagner Armin
Abstract excerpt
Xylem-derived Pinus radiata cell cultures, which can be induced to differentiate tracheary elements (TEs), were transformed with an RNAi construct designed to silence cinnamyl alcohol dehydrogenase (CAD), an enzyme involved in the biosynthesis of monolignols. Quantitative enzymatic CAD measurements revealed reduced CAD activity levels in most transclones generated. TEs from transclones with approximately 20%...
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