Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA.

Abstract excerpt

Using fast Fourier transform (FFT) analysis, we previously observed that cells within Escherichia coli biofilm are organized in nonrandom or periodic spatial patterns. Here, we developed a gravity displacement assay for examining cell adherence and used it to quantitatively monitor the formation of two distinct forms of cell attachment, temporary and permanent, during early biofilm development. Temporarily...

Topics

• Adhesins, Bacterial
• Bacterial Adhesion
• Biofilms
• Escherichia coli K12
• Escherichia coli Proteins
• Gene Deletion
• Glucosyltransferases
• Models, Biological
• Morphogenesis
• Mutagenesis, Insertional
• Mutation
• Polysaccharides, Bacterial
• RNA-Binding Proteins