Development of a Nested PCR Method Targeting a Unique Multicopy Element, ISMap02, for Detection ofMycobacterium aviumsubsp.paratuberculosisin Fecal Samples

Abstract excerpt

This study describes the development of a nested PCR assay that uses a unique element (ISMap02) for Mycobacterium avium subsp. paratuberculosis that is present at six copies within the genome. In addition, the sensitivity of the assay with this element was compared to the sensitivity of detection of the IS900 element in both conventional and real-time PCR assays. The specificity of the ISMap02 element was...

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