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Article

Evidence of diversity within the SnRK1b gene family of Hordeum species.

Genome - 1 Aug 2005

Clark Jeremy S C, Dani Maria, Halford Nigel G, Karp Angela

Abstract excerpt

Adaptor-specific polymerase chain reaction (PCR) was used to amplify 3 different products, termed variant A, B, and C, from the seed-specific class (SnRK1b) of the sucrose nonfermenting-1-related protein kinase gene family (SnRK1) of different Hordeum species and cultivars of barley (Hordeum vulgare). Standard PCR or reverse transcription-PCR (RT-PCR) at a high temperature, using primers that differed by 1 or 2...

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PMID: 16094434
DOI: 10.1139/g05-024

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