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Critical Evaluation of Urine-Based PCR Assay for Diagnosis of Lyme Borreliosis

1 Aug 2005

Abstract excerpt

Many approaches were made in recent years to establish urine PCR as a diagnostic tool for Lyme borreliosis, but results are contradictory. In the present study, a standardized protocol spiking urine from healthy donors with a defined amount of whole Borrelia or Borrelia DNA was established. The development of a nested real-time PCR targeting ospA enabled a highly sensitive and quantitative analysis of these...

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PMID: 16085907
DOI: 10.1128/cdli.12.8.910-917.2005

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