Article
[Genotyping of hepatitis E virus by PCR combining with single restriction endonuclease analysis].
Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Jun 2005
Pan Ning, Dai Xing, Meng Ji-hong, Liu She-lan
Abstract excerpt
OBJECTIVE: To develop a simple method for genotyping of hepatitis E virus (HEV) and to investigate HEV genotype distribution in Nanjing area. METHODS: Twenty-seven full HEV sequences currently-available in GenBank were analyzed with MegAlign and MapDraw programs of DNA STAR software. Degenerate primers were designed and applied to amplify a fragment in HEV ORF1 region. HEV genotypes were determined by the size of...
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