Article
Culture of Neospora caninum in the presence of a Mycoplasma Removal Agent results in the selection of a mutant population of tachyzoites.
Parasitology - 1 Jun 2005
Hudson A, Ellis J T
Abstract excerpt
Mycoplasmas are common contaminants of eukaryotic cells grown in tissue culture. A commercially available Mycoplasma Removal Agent (MRA) was therefore assessed for its effect on tachyzoites of Neospora caninum, in order to determine its suitability for further use in parasite cell cultures. Analyses of tachyzoite and excreted-secreted proteins and antigens by SDS-PAGE and Western blotting show that MRA treatment...
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