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Nucleic Acid Amplification Assays for Detection of La Crosse Virus RNA

1 Apr 2005

Abstract excerpt

We report the development of nucleic acid sequence-based amplification (NASBA) and quantitative real-time reverse transcription (RT)-PCR assays for the detection of La Crosse (LAC) virus in field-collected vector mosquito samples and human clinical samples. The sensitivities of these assays were compared to that of a standard plaque assay in Vero cells. The NASBA and quantitative real-time RT-PCR assays...

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PMID: 15815013
DOI: 10.1128/jcm.43.4.1885-1889.2005

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