Article
Validation of a cotton-specific gene, Sad1, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic cottons.
Plant cell reports - 1 Jun 2005
Yang Litao, Chen Jianxiu, Huang Cheng, Liu Yuhui, Jia Shirong, Pan Liangwen, Zhang Dabing
Abstract excerpt
Genetically modified (GM) cotton lines have been approved for commercialization and widely cultivated in many countries, especially in China. As a step towards the development of reliable qualitative and quantitative PCR methods for detecting GM cottons, we report here the validation of the cotton (Gossypium hirsutum) endogenous reference control gene, Sad1, using conventional and real-time (RT)-PCR methods. Both...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.