Article
[Molecular characterization of genetic defects in hemophilia in Shanghai].
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi - 1 Sept 1997
Liu J, Zhang Y, Wang H, Huang W, Cao W, Wang X, Qu B, Wang H, Shao H, Wang Z, Chen L, Huang W
Abstract excerpt
OBJECTIVE: To detect gene mutations in hemophilia A in Shanghai. METHODS: PCR, denaturing gradient gel electrophoresis (DGGE) and DNA sequencing were used. Fifty Chinese cases of hemophilia A without intron 22 inversion, including 24 severe, 9 moderate and 17 mild cases, were screened. Genomic DNA was amplified using GC-clamped primers covering all the exons and flanking intron regions, excluding the middle...
Topics
- Asian People
- China
- Hemophilia A
- Humans
- Introns
- Mutation