Article
New temperature-sensitive alleles of ftsZ in Escherichia coli.
Journal of bacteriology - 1 Jan 2005
Addinall Stephen G, Small Elaine, Whitaker Duncan, Sturrock Shane, Donachie William D, Khattar Medhat M
Abstract excerpt
We isolated five new temperature-sensitive alleles of the essential cell division gene ftsZ in Escherichia coli, using P1-mediated, localized mutagenesis. The five resulting single amino acid changes (Gly109-->Ser109 for ftsZ6460, Ala129-->Thr129 for ftsZ972, Val157-->Met157 for ftsZ2066, Pro203-->Leu203 for ftsZ9124, and Ala239-->Val239 for ftsZ2863) are distributed throughout the FtsZ core region, and all...
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