Article
Wide-cross whole-genome radiation hybrid mapping of cotton (Gossypium hirsutum L.).
Genetics - 1 Jul 2004
Gao Wenxiang, Chen Z Jeffrey, Yu John Z, Raska Dwaine, Kohel Russell J, Womack James E, Stelly David M
Abstract excerpt
We report the development and characterization of a "wide-cross whole-genome radiation hybrid" (WWRH) panel from cotton (Gossypium hirsutum L.). Chromosomes were segmented by gamma-irradiation of G. hirsutum (n = 26) pollen, and segmented chromosomes were rescued after in vivo fertilization of G. barbadense egg cells (n = 26). A 5-krad gamma-ray WWRH mapping panel (N = 93) was constructed and genotyped at 102 SSR...
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