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New Real-Time Quantitative PCR Procedure for Quantification of Bifidobacteria in Human Fecal Samples

1 Jul 2004

Abstract excerpt

The application of a real-time quantitative PCR method (5' nuclease assay), based on the use of a probe labeled at its 5' end with a stable, fluorescent lanthanide chelate, for the quantification of human fecal bifidobacteria was evaluated. The specificities of the primers and the primer-probe combination were evaluated by conventional PCR and real-time PCR, respectively. The results obtained by real-time PCR...

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PMID: 15240297
DOI: 10.1128/aem.70.7.4165-4169.2004

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