Article
A systemic gene silencing method suitable for high throughput, reverse genetic analyses of gene function in fern gametophytes.
BMC plant biology - 16 Apr 2004
Rutherford George, Tanurdzic Milos, Hasebe Mitsuyasu, Banks Jo Ann
Abstract excerpt
BACKGROUND: Ceratopteris richardii is a useful experimental system for studying gametophyte development and sexual reproduction in plants. However, few tools for cloning mutant genes or disrupting gene function exist for this species. The feasibility of systemic gene silencing as a reverse genetics tool was examined in this study. RESULTS: Several DNA constructs targeting a Ceratopteris protoporphyrin IX...
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