Article
Detection of Cryptosporidium and Giardia in molluscan shellfish by multiplexed nested-PCR.
International journal of food microbiology - 15 Mar 2004
Gómez-Couso H, Freire-Santos F, Amar C F L, Grant K A, Williamson K, Ares-Mazás M E, McLauchlin J
Abstract excerpt
A multiplexed nested-PCR procedure (ABC-PCR) previously developed to detect Cryptosporidium spp. and Giardia duodenalis assemblages A and B in whole human faeces was applied to DNA extracted from filter-feeding molluscs. Species of Cryptosporidium and G. duodenalis were identified by restriction fragment analysis of the PCR products and by DNA sequencing. The extraction and ABC-PCR procedures were shown to be...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
