Article
Simultaneous genotypic and immunophenotypic analysis of interphase cells using dual-color fluorescence: a demonstration of lineage involvement in polycythemia vera.
Blood - 15 Aug 1992
Price C M, Kanfer E J, Colman S M, Westwood N, Barrett A J, Greaves M F
Abstract excerpt
Fluorescent in situ hybridization has become a useful technique by which chromosomal abnormalities may be shown in interphase cells. We present a dual-fluorescence method whereby a chromosomal and immunophenotypic marker can be visualized simultaneously in the same interphase cell. Two patients with the myeloproliferative disorder polycythemia vera and trisomy for chromosome 8 have been studied using this...
Topics
- Aged
- Chromosomes, Human, Pair 8
- Erythrocytes
- Female
- Genotype
- Granulocytes
- Humans
- Immunophenotyping
- Male
- Microscopy, Fluorescence
- Middle Aged