Article
Effects of artificial codon changes in the movement protein gene on adaptation of a hybrid bromovirus to cowpea.
Microbiology and immunology - 1 Jan 2004
Sasaki Nobumitsu, Kaido Masanori, Okuno Tetsuro, Mise Kazuyuki
Abstract excerpt
A hybrid Cowpea chlorotic mottle virus(CCMV) with the movement protein (MP) gene replaced with that of the closely related Brome mosaic virus cannot infect cowpea systemically. Twenty-nine spontaneous mutants from the hybrid CCMV capable of systemic infection in cowpea appeared through biased codon changes that resulted in Lys or Arg at five specific positions in the MP gene. In this study, we report that...
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