Article
Structure of amylase genes in populations of Pacific Cupped oyster ( Crassostrea gigas): tissue expression and allelic polymorphism.
Marine biotechnology (New York, N.Y.) - 1 Jan 2000
Sellos D, Moal J, Degremont L, Huvet A, Daniel J-Y, Nicoulaud S, Boudry P, Samain J-F, Van Wormhoudt A
Abstract excerpt
Using the previously determined complementary DNA Sequence of Crassostrea gigas amylase (Y08370), we designed several oligonucleotide primers and used them with polymerase chain reaction (PCR) technology to characterize oyster amylase gene sequences. Two genes encoding 2 different amylases were characterized and sequenced. The 2 genes are similarly organized with 8 exons and 7 introns. Intron insertions are found...
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