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Development of type I genetic markers from expressed sequence tags in highly polymorphic species.

Marine biotechnology (New York, N.Y.) - 1 Apr 2011

Kim Woo-Jin, Jung Hyungtaek, Gaffney Patrick M

Abstract excerpt

Expressed sequence tag (EST) databases provide a primary source of nuclear DNA sequences for genetic marker development in non-model organisms. To date, the process has been relatively inefficient for several reasons: 1) priming site polymorphism in the template leads to inferior or erratic amplification; 2) introns in the target amplicon are too large and/or numerous to allow effective amplification under...

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PMID: 20309599
DOI: 10.1007/s10126-010-9280-4

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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