Article
Development of type I genetic markers from expressed sequence tags in highly polymorphic species.
Marine biotechnology (New York, N.Y.) - 1 Apr 2011
Kim Woo-Jin, Jung Hyungtaek, Gaffney Patrick M
Abstract excerpt
Expressed sequence tag (EST) databases provide a primary source of nuclear DNA sequences for genetic marker development in non-model organisms. To date, the process has been relatively inefficient for several reasons: 1) priming site polymorphism in the template leads to inferior or erratic amplification; 2) introns in the target amplicon are too large and/or numerous to allow effective amplification under...
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