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Detection of Borrelia burgdorferi in Ticks by Species-Specific Amplification of the Flagellin Gene

1 Dec 1992

Abstract excerpt

We developed a polymerase chain reaction (PCR) that specifically amplifies a fragment of the flagellin gene (fla) of Borrelia burgdorferi, the causative agent of Lyme disease. This fla target, amplified with nested primers, was conserved among all 80 strains of B. burgdorferi tested. Strains examined included cultures from ticks, humans, and rodents from major B. burgdorferi-endemic regions of the United States...

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Identifiers

PMID: 1471733
DOI: 10.4269/ajtmh.1992.47.730

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