Article
Bradyrhizobium elkanii rtxC gene is required for expression of symbiotic phenotypes in the final step of rhizobitoxine biosynthesis.
Applied and environmental microbiology - 1 Jan 2004
Okazaki Shin, Sugawara Masayuki, Minamisawa Kiwamu
Abstract excerpt
We disrupted the rtxC gene on the chromosome of Bradyrhizobium elkanii USDA94 by insertion of a nonpolar aph cartridge. The rtxC mutant, designated DeltartxC, produced serinol and dihydrorhizobitoxine but no rhizobitoxine, both in culture and in planta. The introduction of cosmids harboring the rtxC gene into the DeltartxC mutant complemented rhizobitoxine production, suggesting that rtxC is involved in the final...
Topics
- Bradyrhizobium
- Culture Media
- Fabaceae
- Gene Expression Regulation, Bacterial
- Genetic Complementation Test
- Mutation
- Nitrogen Fixation
- Oxidoreductases
- Phenotype
- Plant Leaves
- Propanolamines
- Glycine max
- Symbiosis
- Transcription, Genetic