Use of the Roche LightCycler Instrument in a Real-Time PCR for <i>Trichomonas vaginalis</i> in Urine Samples from Females and Males

Abstract excerpt

Trichomonas vaginalis is the agent of a highly prevalent sexually transmitted infection (STI) that can result in vaginitis, urethritis, and preterm birth. Traditional methods of diagnosis, including wet preparation, can be unreliable. In this study, we describe the adaptation of an existing PCR method for specific detection of T. vaginalis DNA into a rapid real-time PCR assay based on fluorescence resonance...

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