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Article

Multiplex PCR and minisequencing of SNPs--a model with 35 Y chromosome SNPs.

Forensic science international - 14 Oct 2003

Sanchez Juan J, Børsting Claus, Hallenberg Charlotte, Buchard Anders, Hernandez Alexis, Morling Niels

Abstract excerpt

We have developed a robust single nucleotide polymorphism (SNPs) typing assay with co-amplification of 25 DNA-fragments and the detection of 35 human Y chromosome SNPs. The sizes of the PCR products ranged from 79 to 186 base pairs. PCR primers were designed to have a theoretical Tm of 60 +/- 5 degrees C at a salt concentration of 180 mM. The sizes of the primers ranged from 19 to 34 nucleotides. The...

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PMID: 14550618
DOI: 10.1016/s0379-0738(03)00299-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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