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Rapid, sensitive detection of Mycoplasma pneumoniae in simulated clinical specimens by DNA amplification

1 Dec 1992

Abstract excerpt

The polymerase chain reaction (PCR) was investigated as a means of diagnosing Mycoplasma pneumoniae infections. The target DNA sequence was a 375-bp segment of the P1 virulence protein. This DNA segment was amplified in pure cultures of five different strains of M. pneumoniae but not in other species of Mycoplasma, Acholeplasma, or Ureaplasma that were tested. Simulated clinical specimens were used to compare...

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PMID: 1452717
DOI: 10.1128/jcm.30.12.3280-3283.1992

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