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Evaluation of a new real-time PCR assay for detection of Mycoplasma pneumoniae in clinical specimens.

1 Feb 2012

Abstract excerpt

OBJECTIVE: To establish and evaluate a real-time PCR assay to detect Mycoplasma pneumoniae (M.pneumoniae) in clinical specimens. METHODS: By analysing the whole p1 gene sequence of 60 M.pneumoniae clinical isolates in Beijing of China, an optimized real-time PCR assay (MpP1) using p1 gene conserved region was designed. The specificity and sensitivity of this assay were evaluated and compared with other two...

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PMID: 22424630
DOI: 10.3967/0895-3988.2012.01.011

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