A conjugation procedure for Bdellovibrio bacteriovorus and its use to identify DNA sequences that enhance the plaque-forming ability of a spontaneous host-independent mutant.

Abstract excerpt

Wild-type bdellovibrios are obligate intraperiplasmic parasites of other gram-negative bacteria. However, spontaneous mutants that can be cultured in the absence of host cells occur at a frequency of 10(-6) to 10(-7). Such host-independent (H-I) mutants generally display diminished intraperiplasmic-growth capabilities and form plaques that are smaller and more turbid than those formed by wild-type strains on...

Topics

• Bdellovibrio
• Cloning, Molecular
• Conjugation, Genetic
• DNA Replication
• DNA, Bacterial
• Escherichia coli
• Gene Library
• Genetic Vectors
• Genome, Bacterial
• Mutation
• Phenotype