Utility of internal markers to improve the accuracy of cystic fibrosis genotype analysis.

Article

Utility of internal markers to improve the accuracy of cystic fibrosis genotype analysis.

BioTechniques - 1 Sept 1992

Raskin S, Phillips J A, Vnencak-Jones C, Dawson E, Kaplan G, McClure M

Abstract excerpt

DNA diagnostic tests often utilize restriction endonuclease digestion of PCR-amplified portions of genes under analysis. When partial digestion occurs, the resulting patterns may lead to error in diagnosis. To overcome such potential errors in cystic fibrosis testing, we have developed internal m...

Topics

Cystic Fibrosis
Cystic Fibrosis Transmembrane Conductance Regulator
Genes, Synthetic
Genetic Markers
Genotype
Humans
Membrane Proteins
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.