Article
Message amplification phenotyping (MAPPing)--principles, practice and potential.
Trends in biotechnology - 1 May 1992
Larrick J W
Abstract excerpt
Message amplification phenotyping (MAPPing) is a sensitive polymerase chain reaction (PCR)-based technique for the rapid determination of the mRNA phenotype of small numbers of cells. Isolated mRNA is reverse-transcribed (RT) into DNA, and then the DNA fragments corresponding to proteins or genes...
Topics
- Animals
- Base Sequence
- Biotechnology
- DNA
- Gene Amplification
- Humans
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
- RNA, Messenger