Message amplification phenotyping (MAPPing)--principles, practice and potential.

Abstract excerpt

Message amplification phenotyping (MAPPing) is a sensitive polymerase chain reaction (PCR)-based technique for the rapid determination of the mRNA phenotype of small numbers of cells. Isolated mRNA is reverse-transcribed (RT) into DNA, and then the DNA fragments corresponding to proteins or genes of interest are specifically amplified by PCR. The technique, also called RT-PCR, has wide application in biology and...

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