Article
Molecular cloning of a gene (cfp) encoding the cytoplasmic filament protein P59Nc and its genetic relationship to the snowflake locus of Neurospora crassa.
Genetics - 1 Jul 1992
Haedo S D, Temporini E D, Alvarez M E, Maccioni H J, Rosa A L
Abstract excerpt
P59Nc is a 59-kD polypeptide associated with 8-10-nm diameter cellular filaments in normal Neurospora crassa strains. Abnormally sized and shaped bundles of these structures are present in N. crassa strains carrying mutations at the locus sn (snowflake). By using molecular cloning and restriction fragment length polymorphism (RFLP) segregation analysis strategies we show here that sn is not the genetic locus of...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Centromere
- Chromosome Mapping
- Cloning, Molecular
- DNA, Fungal
- Fungal Proteins
- Genes, Fungal
- Microtubule-Associated Proteins
- Molecular Sequence Data
