Article
Detection of sequence variants in the gene for human type II procollagen (COL2A1) by direct sequencing of polymerase chain reaction-amplified genomic DNA.
Human mutation - 1 Jan 1992
Williams C J, Harrison D A, Hopkinson I, Baldwin C T, Ahmad N N, Ala-Kokko L, Korn R M, Buxton P G, Dimascio J, Considine E L
Abstract excerpt
The direct sequencing of the human type II procollagen (COL2A1) gene from polymerase chain reaction (PCR)-amplified genomic DNA is described. Thirty-two regions of the COL2A1 gene were asymmetrically amplified with intron primers which were specifically chosen to amplify a region spanning 500 to...
Topics
- Alleles
- Base Sequence
- Consensus Sequence
- DNA
- DNA Probes
- Gene Frequency
- Genetic Variation
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Procollagen
- Sequence Analysis, DNA