Article
Role of the human cytomegalovirus major immediate-early promoter's 19-base-pair-repeat cyclic AMP-response element in acutely infected cells.
Journal of virology - 1 Jun 2003
Keller M J, Wheeler D G, Cooper E, Meier J L
Abstract excerpt
Prior studies have suggested a role of the five copies of the 19-bp-repeat cyclic AMP (cAMP)-response element (CRE) in major immediate-early (MIE) promoter activation, the rate-limiting step in human cytomegalovirus (HCMV) replication. We used two different HCMV genome modification strategies to test this hypothesis in acutely infected cells. We report the following: (i) the CREs do not govern basal levels of MIE...
Topics
- Base Pairing
- Cell Line
- Cyclic AMP
- Cytomegalovirus
- Cytomegalovirus Infections
- Gene Expression Regulation, Viral
- Humans
- Immediate-Early Proteins
- Mutation
- Promoter Regions, Genetic
- Repetitive Sequences, Nucleic Acid
- Response Elements
- Transcription, Genetic