Saccharomyces cerevisiae and Saccharomyces paradoxus coexist in a natural woodland site in North America and display different levels of reproductive isolation from European conspecifics.

Abstract excerpt

We report the isolation of multiple strains of Saccharomyces cerevisiae and Saccharomyces paradoxus from a natural woodland site in southeastern Pennsylvania, USA, using enrichment culturing in a medium containing 7.6% (v/v) ethanol. The method was applied to bark and flux material collected from broad-leaved trees (mostly Quercus spp.) and to associated soils. Many candidate wild strains of Saccharomyces were...

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