Identification of the DNA binding sites of PerA, the transcriptional activator of the bfp and per operons in enteropathogenic Escherichia coli.

doi:10.1128/JB.185.9.2835-2847.2003

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Article

Identification of the DNA binding sites of PerA, the transcriptional activator of the bfp and per operons in enteropathogenic Escherichia coli.

Journal of bacteriology - 1 May 2003

Ibarra J Antonio, Villalba Miryam I, Puente José Luis

Abstract excerpt

The bundle-forming pilus (BFP) is an important virulence factor for enteropathogenic Escherichia coli (EPEC). Genes involved in its biogenesis and regulation are tightly regulated by PerA (BfpT), a member of the AraC/XylS family of transcriptional regulators. The aim of this work was to purify PerA and determine its association with bfpA and perA (bfpT) regulatory regions by electrophoretic mobility shift and...

Topics

Base Sequence
Binding Sites
Consensus Sequence
DNA Footprinting
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
Escherichia coli
Escherichia coli Proteins
Fimbriae Proteins
Fimbriae, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Operon

Promoter Regions, Genetic

Repressor Proteins

Sequence Alignment

Transcription, Genetic

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Identifiers

PMID: 12700263
DOI: 10.1128/JB.185.9.2835-2847.2003
PMCID: PMC154397

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